BRAIN Initiative: Scaled reagent resources for brain cell type-specific access across vertebrate species (U01 Clinical Trial Not Allowed)

Funding Agency:
National Institutes of Health

This Notice of Funding Opportunity (NOFO) from the NIH Brain Research through Advancing Innovative Neurotechnologies (BRAIN) Initiative is intended to support the scaling up of brain cell type-specific molecular or genetic access reagents to study circuit function. Scaled engineering and validation of cell type-specific reagents are priorities to be supported.  Applications are sought for Reagent Resource for Design and Development projects that scale up the creation of these tools for several vertebrate species, including in experimental animals and human ex vivo tissues or cells.  Projects will integrate: scaled engineering, validation, cataloguing, and adaptation into easily disseminable formats of reagents. Scaled up projects are sought that will develop tens to hundreds of cell type-selective reagents. Validated reagents produced in these projects are intended to be distributed to neuroscience researchers via centralized Production and Distribution Facilities to be supported separately. This NOFO is part of the BRAIN Initiative Armamentarium project, whose overall goal is to generate tools to specifically access, manipulate, and monitor brain cell types across multiple vertebrate species.  This NOFO will foster close interaction between technologists, disseminators, and neurobiologists in a research consortium including investigators funded by other Armamentarium NOFOs.

This NOFO requires a Plan for Enhancing Diverse Perspectives (PEDP), which will be assessed as part of the scientific and technical peer review evaluation.  Applications that fail to include a PEDP will be considered incomplete and will be withdrawn.

Applicants are strongly encouraged to read the NOFO instructions carefully and view the available PEDP guidance material.


  • Letter of Intent Due Date(s): 30 days prior to the application due date

  • Application Due Date(s): June 14, 2024; Jan. 17, 2025

RFA-MH-25-100 Expiration Date January 18, 2025

Agency Website

Amount Description

Issuing IC and partner components intend to commit an estimated total of $14,000,000 per year to fund 4 to 6 awards.

Application budgets are not limited but need to reflect the actual needs of the proposed project.

Funding Type





Engineering and Physical Sciences
Medical - Basic Science
Medical - Clinical Science

External Deadline

June 14, 2024