Cryo-CLEM Labels RFA

Funding Agency:
Chan Zuckerberg Initiative

The Chan Zuckerberg Initiative (CZI) seeks to support one-year projects in the field of cryo-correlative light and electron microscopy (cryo-CLEM) labels. To gain a deeper mechanistic understanding of biological systems, CZI’s Imaging program aims to visualize and measure them across biological scales and in their biological context. This Request for Applications (RFA) seeks to drive development of complete, general-purpose, intracellular imaging probes for cryo-CLEM. This grant program will advance the utility of cryo-electron tomography (cryo-ET) of cells by providing information on specific aspects of cell function.

Deadline: July 20, 2023

Agency Website

Eligibility Requirements

  • Applications may be submitted by domestic and foreign nonprofit and for-profit organizations, public and private institutions, such as colleges, universities, hospitals, laboratories, units of state and local government, companies, and eligible agencies of the federal government. As part of the application process, for-profit organizations may need to provide additional information on the charitable purposes of the proposal. Grants are not permitted to individuals, only to organizations.
  • There may be more than one application submitted by each organization.
  • Each application should designate one Principal Investigator (PI) as the Coordinating Principal Investigator (Coordinating PI). The Coordinating PI will act as the administrative contact between CZI and all PIs on the grant. The Coordinating PI must submit the application on behalf of all PIs. The Coordinating PI must be affiliated with the institution submitting the application, and grant funds will be awarded to that institution, which will take responsibility for distributing funds to any other institutions. Note that institutions outside the U.S. may not subcontract to U.S. institutions, so please be mindful when selecting the Coordinating PI/institution.
  • Each application should have a minimum of one PI (Coordinating PI), but may designate up to two total PIs (one Coordinating PI and one Co-PI).
  • PIs may only serve as the Coordinating PI on one application, but may serve as a Co-PI on applications different from the one they submitted.
  • Co-PIs may serve as a Co-PI on multiple applications.
  • PIs/Co-PIs on one application may be employed at the same or at different institutions. 
  • PIs and Co-PIs must hold a PhD, MD, or equivalent degree.



Amount Description

  • Award period and start date: Awards will be one year (12 months) in duration with an expected start date of December 1, 2023.
  • Budget: The maximum budget allocated for projects is $165,000 USD in total costs. Proposed budgets should reflect the project scope. $15,000 USD direct costs of the proposed budget must be included as a budget item to support trainees working on the project, beyond personnel costs. Indirect costs cannot exceed 15 percent of direct costs.

Funding Type





Environmental & Life Sciences
Medical - Basic Science

External Deadline

July 20, 2023