Integrated Functional Mapping and Molecular Profiling of Cell Ensembles Encoding the Effects of Addictive Substances in Rodents (R01 Clinical Trial Not Allowed)

Funding Agency:
National Institutes of Health

This notice of funding opportunity (NOFO) aims to support research programs that adopt innovative scalable technologies to inventory, register and molecularly profile distributed cell ensembles encoding the effects of addictive substances, across various stages of exposure (acute, chronic, withdrawal, abstinence, relapse). Emphasis is on approaches capable of integrating data generated through population-scale measurements of cellular activity dynamics in behaving rodents, with other granular data modalities, collected from the same cells at single-cell resolution, such as transcriptomic class, epigenetic state, connectivity and spatial localization. The overarching goal is to inform new models for the mechanisms whereby cells are recruited into neuronal ensembles to encode addiction-associated neurobehavioral states.


  • Letter of Intent Due Date(s):30 days prior to application due date¬†

  • Application Due Date(s): Feb. 21, 2024; Feb. 20, 2025; Feb. 23, 2026

RFA-DA-25-023 Expiration Date February 24, 2026

Agency Website



Amount Description

Applications may not request more than $700,000 direct costs for any one year.

The maximum project period is 5 years.

Funding Type





Environmental & Life Sciences
Medical - Basic Science

External Deadline

February 21, 2024