Technology Development for Single-Molecule Protein Sequencing and Single-Cell Proteome Analysis (R01 Clinical Trial not allowed)

Funding Agency:
National Institutes of Health

This Funding Opportunity Announcement (FOA) solicits grant applications to catalyze major advances in single-molecule protein sequencing and single cell proteome analysis through technology development. The goal of this initiative is to achieve technological advances over the next five years that enable generation of protein sequencing data at sufficient scale, speed, cost and accuracy to use routinely in studies of genome biology and function, and in biomedical and clinical research in general.

Although this funding opportunity uses the R01 mechanism, it runs in parallel with an announcement of identical scientific scope, RFA-HG-21-002, which uses the R21 mechanism. 


  • Letter of Intent Due Date(s): 30 days prior to the application due date

  • Application Due Date(s): October 1, 2021; June 15, 2022; June 15, 2023  

RFA-HG-21-001 Expiration Date New Date December 1, 2022 per issuance of NOT-HG-23-013. (Original Expiration Date: June 16, 2023)

Agency Website



Amount Description

An applicant may request direct costs of up to $500,000 per year. Because the nature and scope of the proposed research will vary from application to application, it is anticipated that the size of each award will also vary.

The scope of the proposed project should determine the project period. The maximum project period is 3 years.

    Funding Type



    Women and minorities encouraged


    Engineering and Physical Sciences
    Environmental & Life Sciences
    Medical - Basic Science
    Medical - Clinical Science
    Medical - Translational

    External Deadline

    June 15, 2022