Technology Development for Single-Molecule Protein Sequencing (R43/R44 Clinical Trial Not Allowed)

Funding Agency:
National Institutes of Health

This Funding Opportunity Announcement (FOA) solicits R43/R44 grant applications to catalyze major advances in single-molecule protein sequencing through technology development. The goal of this initiative is to achieve technological advances over the next five years that enable generation of protein sequencing data at sufficient scale, speed, cost and accuracy to use routinely in studies of genome biology and function, and in biomedical and clinical research in general. Exploration of methods other than those currently being commercialized is highly encouraged.


  • Letter of Intent Due Date(s): 30 days prior to the application due date

  • Application Due Date(s): October 1, 2021; June 15, 2022; June 15, 2023  

PAR-21-247 Expiration Date June 16, 2023

Agency Website

Eligibility Requirements

Only United States small business concerns (SBCs) are eligible to submit applications for this opportunity.



Amount Description


    Funding Type





    Engineering and Physical Sciences
    Environmental & Life Sciences
    Medical - Basic Science
    Medical - Clinical Science
    Medical - Translational

    External Deadline

    June 15, 2023